PVOH and spontaneous Raman-based images of spinal cord in the immediate aftermath of localized contusive injury obtained noninvasively and in vivo in a rat model using remitted light from a single spatially scanned laser

Seth Fillioe; Kyle K. Bishop; Alexander V. S. Jannini; John J. I. Kim; Ricky McDonough; Steven Ortiz; Julie Hasenwinkel; J. H. Henderson; Charles M. Peterson; Joseph Chaiken

doi:10.1117/12.2654526

10 April 2023 PVOH and spontaneous Raman-based images of spinal cord in the immediate aftermath of localized contusive injury obtained noninvasively and in vivo in a rat model using remitted light from a single spatially scanned laser

Seth Fillioe, Kyle K. Bishop, Alexander V. S. Jannini, John J. I. Kim, Ricky McDonough, Steven Ortiz, Julie Hasenwinkel, J. H. Henderson, Charles M. Peterson, Joseph Chaiken

Author Affiliations +

Proceedings Volume 12468, Medical Imaging 2023: Biomedical Applications in Molecular, Structural, and Functional Imaging; 124680E (2023) https://doi.org/10.1117/12.2654526
Event: SPIE Medical Imaging, 2023, San Diego, California, United States

Abstract

We produce 2 kinds of images of spinal cord in the immediate aftermath of a localized contusive injury, noninvasively and in vivo in a rat model using remitted light from a single spatially scanned 830 nm laser. Two different processing algorithms/modalities were employed 1) the PVOH algorithm producing images based on the turbidity of the spinal cord tissue which includes the cerebrospinal fluid (CSF) and 2) Raman spectroscopy which allows images based on pH. Whereas PVOH requires relatively little data processing e.g., no separation of Raman and fluorescence emission for implementation, quantitative use of spontaneous Raman spectra to calculate e.g., pH requires careful separation and accounting for the presence of underlying fluorescence. We utilize an unbiased procedure i.e., no assumptions are made, that an unsupervised machine can execute based on the inherent differences in line widths for pure vibrational and vibronic transitions in fluid media, and the overall effect of heterogenous spectral broadening at physiological temperature. The fundamentally stronger signal to noise ratio for PVOH imaging allows much greater spatial resolution for the same collection times needed to construct the Raman based pH images. This proof of principle study is consistent with a hypothesis that fast localized change in the CSF pH, induced by the mechanical disruption of tissues during the injury, disrupts the delicate balance of chemical factors stabilizing the CSF phase stability. We suggest that this combination of new methodologies for real-time imaging of chemical and physical changes in spinal cords and other tissues could have many uses.

Conference Presentation

Citation Download Citation

Seth Fillioe, Kyle K. Bishop, Alexander V. S. Jannini, John J. I. Kim, Ricky McDonough, Steven Ortiz, Julie Hasenwinkel, J. H. Henderson, Charles M. Peterson, and Joseph Chaiken "PVOH and spontaneous Raman-based images of spinal cord in the immediate aftermath of localized contusive injury obtained noninvasively and in vivo in a rat model using remitted light from a single spatially scanned laser", Proc. SPIE 12468, Medical Imaging 2023: Biomedical Applications in Molecular, Structural, and Functional Imaging, 124680E (10 April 2023); https://doi.org/10.1117/12.2654526

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available