Cell death mechanisms induced by green synthesized silver nanoparticles in combination with pheophorbide a-mediated photodynamic therapy against resistant MCF-7 cells overexpressed with P-glycoprotein

Alexander Chota; Blassan P. George; Heidi Abrahamse

doi:10.1117/12.2675917

11 August 2023 Cell death mechanisms induced by green synthesized silver nanoparticles in combination with pheophorbide a-mediated photodynamic therapy against resistant MCF-7 cells overexpressed with P-glycoprotein

Alexander Chota, Blassan P. George, Heidi Abrahamse

Author Affiliations +

Proceedings Volume 12627, Translational Biophotonics: Diagnostics and Therapeutics III; 126270V (2023) https://doi.org/10.1117/12.2675917
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Globally, multidrug resistance (MDR) in breast cancer has become the major cause of morbidity and mortality among women. This study was designed to overcome resistance, reduce dose-dependence in photodynamic therapy (PDT) and evaluate cell death mechanisms induced by green synthesized silver nanoparticles (AgNPs) in combination with pheophorbide-a mediated PDT on superlative, and most architectured three-dimensional (3-D) doxorubicin (DOX) resistant MCF-7 breast cancer cells with overexpressed p-glycoproteins in vitro. In addition to the aforementioned scope, the combination of green NPs with PDT has been reported to yield a good disease prognosis which in most cases is accompanied with manageable adverse effects. Briefly, MDR MCF-7 breast cancer cells were cultured in a 96 well plate to form 3D tumor spheroids and later treated with optimized concentrations of AgNPs and pheophorbide-a in monotherapy. After 24 h treatment, 3-[4,5-dimethylthiazole-2- yl]-2,5- diphenyl tetrazolium bromide (MTT) assay was performed to determine the 50% inhibitory concentration (IC₅₀) for both experimental models. Morphological changes were observed by using an inverted light microscope, viability by MTT assay, and cell death analysis by Annexin VFITC-PI staining. Taken together, the results from this study displayed a dose-dependent decrease in cell viability which was accompanied by significant morphological changes. Furthermore, Annexin V-FITC-PI assay showed apoptosis as the most prominent cell death mechanism induced by PPBa-mediated PDT and AgNPs. Taken together, the findings from the present study highlight the advantages of green nanotechnology in cancer therapy.

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Alexander Chota, Blassan P. George, and Heidi Abrahamse "Cell death mechanisms induced by green synthesized silver nanoparticles in combination with pheophorbide a-mediated photodynamic therapy against resistant MCF-7 cells overexpressed with P-glycoprotein", Proc. SPIE 12627, Translational Biophotonics: Diagnostics and Therapeutics III, 126270V (11 August 2023); https://doi.org/10.1117/12.2675917

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KEYWORDS

Photodynamic therapy

Cell death

Cancer

3D modeling

Nanoparticles

Medical research

Silver

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