Characterization of lipid components in human cells by means of ATR FT-IR spectroscopy

Bahar Faramarzi; Marianna Portaccio; Ines Delfino; Maria Lepore

doi:10.1117/12.2670762

11 August 2023 Characterization of lipid components in human cells by means of ATR FT-IR spectroscopy

Bahar Faramarzi, Marianna Portaccio, Ines Delfino, Maria Lepore

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Proceedings Volume 12627, Translational Biophotonics: Diagnostics and Therapeutics III; 126272Q (2023) https://doi.org/10.1117/12.2670762
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

The study of lipid components in human cells is of fundamental importance in many cellular functions, such as cell adhesion and migration, formation of membrane domain, DNA damage response, senescence, ageing autophagy, and apoptosis. For this reason, we investigated the different phospholipids and sphingolipids components of human cells by using Fourier Transform Infrared (FT-IR) spectroscopy that allows lipids detection and their characterization in biological samples. Commercial samples of phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylserine (PS), ceramide (Cer), ceramide 1-phosphate (C1P), sphingosine 1-phosphate (S1P) and sphingomyelin (SM) were used for collecting spectra using ATR acquisition mode. The infrared spectra of different lipids show the contribution of various functional groups from hydrocarbon chains and polar head groups. The present analysis of these spectra contributed to a better understanding of the characteristics of infrared spectra of single lipid components that can be considered a preliminary step in the FT-IR characterization of lipids extracts from human cells affected by pathologies or exposed to different external agents.

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Bahar Faramarzi, Marianna Portaccio, Ines Delfino, and Maria Lepore "Characterization of lipid components in human cells by means of ATR FT-IR spectroscopy", Proc. SPIE 12627, Translational Biophotonics: Diagnostics and Therapeutics III, 126272Q (11 August 2023); https://doi.org/10.1117/12.2670762

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KEYWORDS

FT-IR spectroscopy

Attenuated total reflectance

Biological samples

Infrared radiation

Infrared spectroscopy

Pathology

Picosecond phenomena

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