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28 September 2023 Metasurface-based optical system for the generation of Bessel beams with independent optical parameters in fluorescence microscopy
Anna Archetti, Matteo Bruzzone, Giulia Tagliabue, Marco dal Maschio
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Proceedings Volume 12665, Novel Optical Systems, Methods, and Applications XXVI; 1266508 (2023) https://doi.org/10.1117/12.2675733
Event: SPIE Optical Engineering + Applications, 2023, San Diego, California, United States
Abstract
Light shaping approaches are fundamental techniques adopted in a variety of applications such as material processing, communication, and microscopy. Here, we present a method based on wave front engineering to generate 3D arrangements of Bessel Beams (BB) with independent optical properties all encoded in one single, flat, and lightweight optical element. We fabricated and characterized a Silicon Nitride (SiNx) element using Meta-Surface (MS) technology to generate one of the designed BB lattices. We demonstrated its application in microscopy by integrating it along the excitation path of a light-sheet microscope (LSM) and recording neuronal activity from the zebrafish larva brain.
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Anna Archetti, Matteo Bruzzone, Giulia Tagliabue, and Marco dal Maschio "Metasurface-based optical system for the generation of Bessel beams with independent optical parameters in fluorescence microscopy", Proc. SPIE 12665, Novel Optical Systems, Methods, and Applications XXVI, 1266508 (28 September 2023); https://doi.org/10.1117/12.2675733
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KEYWORDS
Bessel beams
Fluorescence microscopy
Light sources and illumination
Optical microscopy
Optical properties
Optical resolution
Optical simulations
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