Detection of Der p1 dust mite allergen sIgE based on microfluidic chip and digital ELISA

Ju Xue; Shih-Mo Yang

doi:10.1117/12.2687635

28 August 2023 Detection of Der p1 dust mite allergen sIgE based on microfluidic chip and digital ELISA

Ju Xue, Shih-Mo Yang

Proceedings Volume 12724, Second International Conference on Biomedical and Intelligent Systems (IC-BIS 2023); 127241I (2023) https://doi.org/10.1117/12.2687635
Event: Second International Conference on Biomedical and Intelligent Systems (IC-BIS2023), 2023, Xiamen, China

Abstract

Indoor dust mites are one of the common and major inhalant allergens, and detection of specific antibodies against dust mite allergens can help in the prevention and treatment of allergic diseases and in the study of immunotherapy. However, traditional assays such as enzyme-linked immunosorbent assays are time-consuming and costly, and are not conducive to rapid detection. Microfluidic chips enable precise control and manipulation of tiny fluids, allowing for rapid, low-cost, high-throughput bioassays. Digital enzyme-linked immunosorbent assays have the advantages of high sensitivity and specificity. In this paper, fluorescence-encoded micro-magnetic beads were used as solid-phase carriers to modify Der p1 dust mite antigen, and then combined with patient serum and detection antibody to form an immunosandwich structure. Then, we successfully detected Der p1 dust mite allergen-specific antibodies in patient sera using a combination of microfluidic chip and digital ELISA, and plotted the standard curve. The method has promising applications and can provide an essential reference for clinical diagnosis and treatment.

Citation Download Citation

Ju Xue and Shih-Mo Yang "Detection of Der p1 dust mite allergen sIgE based on microfluidic chip and digital ELISA", Proc. SPIE 12724, Second International Conference on Biomedical and Intelligent Systems (IC-BIS 2023), 127241I (28 August 2023); https://doi.org/10.1117/12.2687635

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