Comparative analysis of photoacoustic signals of immune cells in the context of drug treatment

Sila Koksal; Elif Yaprak Sarac; Zulal Celik; Berkay Mutlu; Ali Can Sahin; Ceren Ciraci; Özgür Özdemir

doi:10.1117/12.3029522

20 June 2024 Comparative analysis of photoacoustic signals of immune cells in the context of drug treatment

Sila Koksal, Elif Yaprak Sarac, Zulal Celik, Berkay Mutlu, Ali Can Sahin, Ceren Ciraci, Özgür Özdemir

Proceedings Volume 13006, Biomedical Spectroscopy, Microscopy, and Imaging III; 130060S (2024) https://doi.org/10.1117/12.3029522
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Valproic acid (VPA) is an epigenetic regulator used in the treatment of epilepsy and other neurological disorders for the past 50 years. The direct impact of VPA on cells of the immune system has only been explored recently. Immune cells have been relatively less studied in the context of photoacoustic tomography imaging, which has been used in different biological systems with many different applications in biomedical investigations. Therefore, we aimed to perform a comparative analysis of photoacoustic imaging of immune cells in the context of VPA treatment. Four different doses of VPA (1 mM, 2.5 mM, 5 mM, and 7.5 mM) were applied to in vitro models established with human EoL-1 eosinophilic cell lines and human Jurkat T lymphocyte cell lines. Cell viability was assessed by a trypan blue exclusion test. The changes in morphology caused by VPA were examined with a photoacoustic tomography system. Photoacoustic signals measured from the VPA in different immune cells showed similar results obtained from light microscopy and cell viability. Photoacoustic imaging is promising for identifying and distinguishing different cell types and detecting changes at the cellular level.

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Citation Download Citation

Sila Koksal, Elif Yaprak Sarac, Zulal Celik, Berkay Mutlu, Ali Can Sahin, Ceren Ciraci, and Özgür Özdemir "Comparative analysis of photoacoustic signals of immune cells in the context of drug treatment", Proc. SPIE 13006, Biomedical Spectroscopy, Microscopy, and Imaging III, 130060S (20 June 2024); https://doi.org/10.1117/12.3029522

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