Monitoring antibiotic susceptibility of Escherichia coli through analysis of fluorescence

Kiran Philip Isaac; Sujatha Narayanan Unni

doi:10.1117/12.3022408

20 June 2024 Monitoring antibiotic susceptibility of Escherichia coli through analysis of fluorescence

Kiran Philip Isaac, Sujatha Narayanan Unni

Proceedings Volume 13006, Biomedical Spectroscopy, Microscopy, and Imaging III; 130061H (2024) https://doi.org/10.1117/12.3022408
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

This study investigates an indirect measurement of the antibiotic susceptibility of Escherichia coli (E. coli) strain ATCC 25922 by analyzing sample background fluorescence at 455 nm excitation and 530 nm emission wavelengths. The study takes a practical approach by exposing the bacterial culture, suspended in Luria-Bertani (LB) broth medium, to various concentrations of ampicillin and analyzing the corresponding fluorescence intensities of the inhibitory and non-inhibitory ranges of antibiotic concentrations. The variations in fluorescence intensities indicate probable antibiotic sensitivity. The results are validated with gold standards such as the broth microdilution method.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Kiran Philip Isaac and Sujatha Narayanan Unni "Monitoring antibiotic susceptibility of Escherichia coli through analysis of fluorescence", Proc. SPIE 13006, Biomedical Spectroscopy, Microscopy, and Imaging III, 130061H (20 June 2024); https://doi.org/10.1117/12.3022408

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