Development of smart nano-kit for rapid, automated, and ultra-sensitive detection of pesticide traces in agricultural samples

Sujatha Narayanan Unni; Rohit Kumar Sharma; Nishima Wangoo; Maitrayee Trivedi; Kavini V; Sudha Karthik; Vishal Balaji

doi:10.1117/12.3029508

17 April 2024 Development of smart nano-kit for rapid, automated, and ultra-sensitive detection of pesticide traces in agricultural samples

Sujatha Narayanan Unni, Rohit Kumar Sharma, Nishima Wangoo, Maitrayee Trivedi, Kavini V, Sudha Karthik, Vishal Balaji

Author Affiliations +

Proceedings Volume 13108, Women in Optics and Photonics in India 2023; 131080M (2024) https://doi.org/10.1117/12.3029508
Event: Women in Optics and Photonics in India 2023, 2024, Chennai, India

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Abstract

In today’s world, pesticides are often used in agriculture to reduce post-harvest losses due to contamination and increase productivity. Long term exposure of these pesticides in food lead to various health issues in humans and animals. An ultrasensitive detection of these pesticides traces in agricultural samples are currently in need. In this work, we have demonstrated a rapid, automated and ultrasensitive detection of organophosphorus pesticide in presence of citrate capped AuNPs, aptamer and cationic polymer Polydiallyldimethylammoniumchloride (PDDA). The instrumentation setup consists of an automatic reagent dispenser and a sample chamber associated with optical devices for absorption measurements. Electrostatic interaction between PDDA and aptamer prevents the aggregation of AuNPs whereas no such inhibition is observed in presence of aptamer specific pesticide, thus changing the colour of the AuNPs from red to blue. The limit of detection and the resolution of the developed equipment is 1pM and 500pM respectively.

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Sujatha Narayanan Unni, Rohit Kumar Sharma, Nishima Wangoo, Maitrayee Trivedi, Kavini V, Sudha Karthik, and Vishal Balaji "Development of smart nano-kit for rapid, automated, and ultra-sensitive detection of pesticide traces in agricultural samples", Proc. SPIE 13108, Women in Optics and Photonics in India 2023, 131080M (17 April 2024); https://doi.org/10.1117/12.3029508

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