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27 August 2024 Initial identification and analysis of the gene responsible for 2-hydroxyisoflavanone dehydratasedase in tobacco (Nicotiana tabacum) and its expression pattern
Chuisi Kong, Zhenchong Wang, Qin Yan, Yao Lu, Guoyou He, Zhufeng Shi, Tao Yang
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Proceedings Volume 13252, Fourth International Conference on Biomedicine and Bioinformatics Engineering (ICBBE 2024); 1325219 (2024) https://doi.org/10.1117/12.3044096
Event: 2024 Fourth International Conference on Biomedicine and Bioinformatics Engineering (ICBBE 2024), 2024, Kaifeng, China
Abstract
The complete mRNA sequence of a specific gene in tobacco (Nicotiana tabacum), known as 2-hydroxyisoflavanone dehydratase, was amplified using rapid amplification of cDNA ends (RACE) methods. The mRNA produced by the tobacco 2-hydroxyisoflavanone dehydratasedase gene was determined to have a length of 1,278bp, and it contains an open reading frame of 966 bp that codes for a protein consisting of 321 amino acids. After conducting sequence analysis, it was found that the tobacco 2-hydroxyisoflavanone dehydratasedase exhibits significant similarity to comparable genes in other plant species, including nicotiana tomentosiformis (99%), capsicum annuum (78%), potato (75%), lycopersicon pennellii (73%) and lycopersicon esculentum (72%). Upon further analysis of the tobacco genomic sequences database, it was discovered that this specific gene does not contain any introns and is composed of a single exon. Furthermore, our results suggest a closer genetic relationship between the 2-hydroxyisoflavanone dehydratasedase gene in tobacco and its equivalent in nicotiana tomentosiformis. Furthermore, tissue expression profile analysis demonstrated high expression levels of this gene in both flower and leaf tissues, while moderate expression was noted in both the root and stem tissues. This experiment serves as an important foundation for future investigations into the functions and characteristics of this specific gene in tobacco.
(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
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Chuisi Kong, Zhenchong Wang, Qin Yan, Yao Lu, Guoyou He, Zhufeng Shi, and Tao Yang "Initial identification and analysis of the gene responsible for 2-hydroxyisoflavanone dehydratasedase in tobacco (Nicotiana tabacum) and its expression pattern", Proc. SPIE 13252, Fourth International Conference on Biomedicine and Bioinformatics Engineering (ICBBE 2024), 1325219 (27 August 2024); https://doi.org/10.1117/12.3044096
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KEYWORDS
Tissues
Proteins
Genetics
Agriculture
Annealing
Organisms
Biological research
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