Reconstruction of surfaces from optical sections

Jill Gemmill; Kenneth R. Sloan Jr.; Christine A. Curcio

doi:10.1117/12.59600

26 June 1992 Reconstruction of surfaces from optical sections

Jill Gemmill, Kenneth R. Sloan Jr., Christine A. Curcio

Author Affiliations +

Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59600
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States

Abstract

We are working on several projects which have in common the reconstruction of smooth surfaces based on data gathered using optical sections. Data collection is done using a great many techniques -- most of which require manual tracing or labelling by a human trained observer. These include: camera lucida drawings or EM photomicrographs traced on a digitizing tablet, and contours traced directly on a frame-grabbed image. Optical sections are acquired using both confocal microscopy and Nomarski optics. Objects of interest range from individual cells (photoreceptors, ganglion cells), through small collections of cells, to extended regions. Some of the objects are fairly simple (e.g., individual cones in human retina, blob- or sheet-like regions of interest); the information is contained in precise measurements of the geometry. Other objects (e.g., dendritic trees) are more complicated, with most of the information contained in the shape of a branching structure. All of these types of data are processed into common representation which produces as its final product a smooth 3-D surface, with arbitrary branching structure. Common tasks such as filtering hand drawn features, automatic alignment of multiple 2-D sections to create a 3-D volume, surface reconstruction, display and analysis are supported.

Citation Download Citation

Jill Gemmill, Kenneth R. Sloan Jr., and Christine A. Curcio "Reconstruction of surfaces from optical sections", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59600

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE