Universal phosphorescence immunoassay

Ekaterina Yu. Mantrova; Marina V. Demcheva; Alexander P. Savitsky; Gely V. Ponomarev

doi:10.1117/12.144715

18 May 1993 Universal phosphorescence immunoassay

Ekaterina Yu. Mantrova, Marina V. Demcheva, Alexander P. Savitsky, Gely V. Ponomarev

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144715
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Pd-coproporphyrin I (Pd-CP) has optimum phosphorescence characteristics for application in immunoassay. The aim of this study is to work out a universal phosphorescence immunoassay method (UniPhIA) using monoclonal antibodies to Pd-CP and conjugates of various proteins with Pd-CP for detection of insulin. Pd-CP and monoclonal antibodies obtained allow a convenient method for determination of various antigens to be developed, which combines the universal character of the amplified enzyme-linked immunosorbent assay (a-ELISA) with a high sensitivity and the simplicity of the time-resolved phosphorescence immunoassay.

Citation Download Citation

Ekaterina Yu. Mantrova, Marina V. Demcheva, Alexander P. Savitsky, and Gely V. Ponomarev "Universal phosphorescence immunoassay", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144715

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