Detection and analysis of leptospiral DNA in early Leptospirosis by polymerase chain reaction and DNA hybridization with Digoxingenin-AMPPD

Lang Bao; Ye-Rong Yu; W. J. Terpstra

doi:10.1117/12.180795

21 July 1994 Detection and analysis of leptospiral DNA in early Leptospirosis by polymerase chain reaction and DNA hybridization with Digoxingenin-AMPPD

Lang Bao, Ye-Rong Yu, W. J. Terpstra

Author Affiliations +

Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180795
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Fourteen serum specimens from patients with early Leptospirosis proven by blood culture and serological test were detected by PCR with the oligonucleotide primers obtained from a genomic library of leptospira interrogans. The amplified DNA were hybridized with the homologous DNA probe labeling with Digoxingenin-AMPPD. All of the samples revealed the presence of leptospira and the strong signals were visualized with homologous DNA probes hybridization. Negative and positive controls appeared correctly. The DNA fragment generated from PCR amplification homologically hybridized with the DNA of 16 strains of leptospira. The single recognized band (about 400 bps) from 6 out of the 16 strains has come out which are representative of the principal strains in Sichuan, China. The results demonstrated that PCR is an advanced diagnostic technique for early Leptospirosis. The treatment of samples is easy and has little risk of DNA loss and contamination. This is a considerable advantage over other detective techniques and can be available especially in China and other developing countries.

Citation Download Citation

Lang Bao, Ye-Rong Yu, and W. J. Terpstra "Detection and analysis of leptospiral DNA in early Leptospirosis by polymerase chain reaction and DNA hybridization with Digoxingenin-AMPPD", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); https://doi.org/10.1117/12.180795

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available