Simultaneous image and point spread function reconstruction for 3D light microscopy

Timothy J. Holmes; Santosh Bhattacharyya; Joshua A. Cooper; David K. Hanzel; Vijaykumar Krishnamurthi; Wen-Chieh Lin; Badrinath Roysam; Donald H. Szarowski; James N. Turner

doi:10.1117/12.188054

30 September 1994 Simultaneous image and point spread function reconstruction for 3D light microscopy

Timothy J. Holmes, Santosh Bhattacharyya, Joshua A. Cooper, David K. Hanzel, Vijaykumar Krishnamurthi, Wen-Chieh Lin, Badrinath Roysam, Donald H. Szarowski, James N. Turner

Author Affiliations +

Proceedings Volume 2302, Image Reconstruction and Restoration; (1994) https://doi.org/10.1117/12.188054
Event: SPIE's 1994 International Symposium on Optics, Imaging, and Instrumentation, 1994, San Diego, CA, United States

Abstract

Blind deconvolution algorithms are being developed for reconstructing (deblurring) 2D and 3D optically sectioned light micrographs, including widefield fluorescence, transmitted light brightfield and confocal fluorescence micrographs. The blind deconvolution concurrently reconstructs the point spread function (PSF) with the image data. This is important because it obviates the necessity to measure the PSF. The PSF measurement is esoteric and sometimes impossible and it thereby will hinder wide routine biological and clinical usage. The iterative algorithms are primarily based on a stochastic model of the physics of fluorescence quantum photons and the optimization criterion of maximum likelihood estimation (MLE), as extended from precursory nuclear medicine MLE algorithms. The algorithm design is mostly model based, although it contains some non- model-based components which have important practical benefits.

Citation Download Citation

Timothy J. Holmes, Santosh Bhattacharyya, Joshua A. Cooper, David K. Hanzel, Vijaykumar Krishnamurthi, Wen-Chieh Lin, Badrinath Roysam, Donald H. Szarowski, and James N. Turner "Simultaneous image and point spread function reconstruction for 3D light microscopy", Proc. SPIE 2302, Image Reconstruction and Restoration, (30 September 1994); https://doi.org/10.1117/12.188054

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available