Lifetime-based sensing in highly scattering media

Henryk Szmacinski; Joseph R. Lakowicz

doi:10.1117/12.208549

8 May 1995 Lifetime-based sensing in highly scattering media

Henryk Szmacinski, Joseph R. Lakowicz

Author Affiliations +

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208549
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Fluorescence lifetime-based sensing is now recognized as a valuable methodology in clinical and analytical chemistry. For clinical chemistry, or for non-invasive sensing through skin, it is often necessary to obtain quantitative information in highly scattering media. Lifetime-based sensing can be used to obtain quantitative measurements in turbid media. We describe frequency-domain lifetime measurements in intralipid suspension, and present a reliable technique which allows phase-modulation lifetime measurements of fluorophores dispersed within or localized within intralipid. Lifetimes can be measured using an intensity decay law which accounts for the time delays and pulse-broadening effects of multiple light scattering events occurring in the intralipid. Alternatively, the phase and modulation measurements can be performed relative to a reference fluorophore of known lifetime. This approach provided reliable lifetime data for a pH-sensitive fluorophore contained within a microcuvette 4 mm under the surface of an intralipid suspension.

Citation Download Citation

Henryk Szmacinski and Joseph R. Lakowicz "Lifetime-based sensing in highly scattering media", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208549

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