Volumetric Raman spectral imaging with a confocal Raman microscope: image modalities and applications

Colin J. H. Brenan; Ian Warwick Hunter; Michael J. Korenberg

doi:10.1117/12.237470

10 April 1996 Volumetric Raman spectral imaging with a confocal Raman microscope: image modalities and applications

Colin J. H. Brenan, Ian Warwick Hunter, Michael J. Korenberg

Proceedings Volume 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III; (1996) https://doi.org/10.1117/12.237470
Event: Electronic Imaging: Science and Technology, 1996, San Jose, CA, United States

Abstract

The Fourier transform Confocal Raman Microscope (FT-CRM) enables non-invasive 3D Raman spectroscopic analysis and visualization of chemically heterogeneous preparations. The instrument combines a confocal optical microscope with a visible light Fourier transform Raman spectrometer to acquire and analyze the Raman spectrum of light scattered from a voxel in the sample defined by the confocal optics. Scanning the sample relative to the confocal voxel and recording the Raman spectrum at each scan position generates a multi- dimensional data set encoding the spatially-varying compositional properties of the sample. We report here on the spatial and spectral FT-CRM image properties that includes recent work on correlation-based Raman spectroscopic imaging and application of parametric spectral estimators for robust Raman spectrum estimation.

Citation Download Citation

Colin J. H. Brenan, Ian Warwick Hunter, and Michael J. Korenberg "Volumetric Raman spectral imaging with a confocal Raman microscope: image modalities and applications", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); https://doi.org/10.1117/12.237470

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