Photonic effects on mononuclear leukocytes

Xiaoyan Dai; Richard B. Beard; Michael A. DellaVecchia M.D.; Alexander V. Priezzhev

doi:10.1117/12.239517

10 May 1996 Photonic effects on mononuclear leukocytes

Xiaoyan Dai, Richard B. Beard, Michael A. DellaVecchia M.D., Alexander V. Priezzhev

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239517
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

The effects of pulsed light (2 Hz) with a 55% duty ratio and continuous light on the migration of human mononuclear leukocytes, MNLs (monocytes and lymphocytes) are reported for red light ((lambda) equals 660 nm) and green light ((lambda) equals 565 nm). The comparison of the relative value of the distance to blood cell migration under light to the control cell migration without light stimulus is recorded as cytokinetic index, K.I. K.I. is a measure of the cytokinesis which is the progress of the cell movement in which the migration is enhanced by substances in the cell environment irrespective of a concentration gradient. Red light stimulation produces K.I.'s for PMNs which are 30% grater than for MNLs. Green light stimulation produces K.I.'s for PMNs less than 1.0 indicative of inhibited migration, while for MNLs the K.I.'s are slightly greater than 1.0 indicative of enhanced migration.

Citation Download Citation

Xiaoyan Dai, Richard B. Beard, Michael A. DellaVecchia M.D., and Alexander V. Priezzhev "Photonic effects on mononuclear leukocytes", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239517

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