Quantitative analysis of anthracyclines kinetics in cells varying in drug responsiveness

Alberto Colasanti; Annamaria Kisslinger; Raffaele Liuzzi; Michele Mastrocinque; Maria Quarto; Patrizia Riccio; Giuseppe Roberti; Fulvia Villani

doi:10.1117/12.260777

4 December 1996 Quantitative analysis of anthracyclines kinetics in cells varying in drug responsiveness

Alberto Colasanti, Annamaria Kisslinger, Raffaele Liuzzi, Michele Mastrocinque, Maria Quarto, Patrizia Riccio, Giuseppe Roberti, Fulvia Villani

Author Affiliations +

Proceedings Volume 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II; (1996) https://doi.org/10.1117/12.260777
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

A fluorimetric technique to measure transport parameters of fluorescent drugs through cellular membranes is described. This method eliminates procedures that would lead to errors in the measurement of drug accumulated by cells, and measures the fraction of free drug in cells. The use of a simple three-compartment model in conjunction with fluorescence measurements performed on the extracellular medium and on Triton-permeabilized cells at different times during daunorubicin incorporation allows determination of the kinetic parameters of the transport through cellular membranes. With this technique we found that LoVo cells have a greater daunorubicin uptake, a similar input rate constant and a lower output rate constant than the drug resistant LoVo/DX cells. Preliminary photoactivation measurements of these two cell lines with these compounds showed that phototoxic effects are related to the amount of drug bound to cellular sites.

Citation Download Citation

Alberto Colasanti, Annamaria Kisslinger, Raffaele Liuzzi, Michele Mastrocinque, Maria Quarto, Patrizia Riccio, Giuseppe Roberti, and Fulvia Villani "Quantitative analysis of anthracyclines kinetics in cells varying in drug responsiveness", Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); https://doi.org/10.1117/12.260777

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available