Irradiation with HeNe laser can influence the cytotoxic response of HeLa cells to ionizing radiation

Tiina I. Karu; Ludmila V. Pyatibrat; G. Kalendo

doi:10.1117/12.260004

25 November 1996 Irradiation with HeNe laser can influence the cytotoxic response of HeLa cells to ionizing radiation

Tiina I. Karu, Ludmila V. Pyatibrat, G. Kalendo

Proceedings Volume 2929, Effects of Low-Power Light on Biological Systems II; (1996) https://doi.org/10.1117/12.260004
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

A monolayer of HeLa cells in plateau-phase of growth was exposed to He-Ne laser radiation either 5 min, 60 min or 180 min before (gamma) -irradiation. It has been shown that in the case of a 5-min interval between the two types of irradiations the survival curve was practically identical to the survival curve of the cells after (gamma) -irradiation only. In the case of He-Ne laser exposure 60 min before (gamma) -irradiation with doses of over 5 Gy, a fraction of more resistant cells was revealed: their D₀ was twice as high as the D₀ of the min population. A preexposure of cells to He-Ne laser 60 or 180 min before (gamma) - irradiation also changed the shape of the growth curves as compared to (gamma) -irradiated cells. It is proposed that the preexposure of cells to He-Ne laser radiation activates, in a subpopulation of cells, processes which speed up the repair of (gamma) -radiation damage. The possibility that the described phenomenon is an adaptive response of cells is discussed.

Citation Download Citation

Tiina I. Karu, Ludmila V. Pyatibrat, and G. Kalendo "Irradiation with HeNe laser can influence the cytotoxic response of HeLa cells to ionizing radiation", Proc. SPIE 2929, Effects of Low-Power Light on Biological Systems II, (25 November 1996); https://doi.org/10.1117/12.260004

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