Oxyhemoglobin measurement of whole blood specimens in a silicon microfabricated cuvette

Caicai Wu; Mark R. Holl; Margaret Kenny; Paul Yager

doi:10.1117/12.269966

31 March 1997 Oxyhemoglobin measurement of whole blood specimens in a silicon microfabricated cuvette

Caicai Wu, Mark R. Holl, Margaret Kenny, Paul Yager

Author Affiliations +

Proceedings Volume 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications; (1997) https://doi.org/10.1117/12.269966
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

The purpose of this study was to develop a miniaturized CO- oximeter for hemoglobin derivative measurement using microfabrication technology. A microcuvette (volume equals 507 nl) was fabricated for analysis of percent oxyhemoglobin (O₂Hb%) in whole blood. A cuvette of 50 micrometer pathlength produced optimal absorbance sensitivity to changes in O₂Hb%. The pressure differential for a nominal blood flow rate of approximately 1 microliter/second was 4.1 kPa (16.6 in water, 0.6 psi). Entrained bubbles were easily discharged at these pressures. Spectral measurements were made using an ocean optics miniaturized spectrophotometer (500 - 700 nm). A fiber optic probe with one receiving and six emitting fibers (200 micrometer core and 0.22 NA) was used for spectral measurement. Heparinized fresh blood from a healthy volunteer was tonometered with N₂, CO₂, and O₂ mixtures to produce six samples with O₂Hb% from 22 - 97%. Chemometrics was used for data analysis. The second derivatives of spectra were taken to eliminate baseline changes caused by RBC light scattering. Indirect calibration by principal component regression was applied to the second derivative. Four factor cross validation showed a correlation coefficient of 0.9994 between measured O₂Hb% of lysed blood using an OSM3 CO- oximeter (Radiometer America, Ohio) and whole blood using the microfabricated cuvette. The linear relationship is: O₂Hb%micro-cuvette equals 0.8411% plus 0.9882 multiplied by O₂Hb%_OSM3. We conclude that O₂Hb% measurement on unlysed whole blood using a silicon microfabricated cuvette is practical and that results are similar to traditional CO- oximetry.

Citation Download Citation

Caicai Wu, Mark R. Holl, Margaret Kenny, and Paul Yager "Oxyhemoglobin measurement of whole blood specimens in a silicon microfabricated cuvette", Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997); https://doi.org/10.1117/12.269966

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