Raman spectroscopy for in situ- evaluation of high-grade malignant gliomas induced in SCID mice

Candace E. Clary; Alex Yu. Dergachev; Sergey B. Mirov; G. Yancey Gillespie

doi:10.1117/12.273517

7 May 1997 Raman spectroscopy for in situ- evaluation of high-grade malignant gliomas induced in SCID mice

Candace E. Clary, Alex Yu. Dergachev, Sergey B. Mirov, G. Yancey Gillespie

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273517
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Each year, more people at younger ages are diagnosed with primary brain tumors. Current histological discrimination between normal and diseased tissue occurs after tissue excision. A reliable optical biopsy for open craniotomy would optimize the amount and types of tissue removal by making an accurate evaluation before excision. The presented work is part of a study investigating the clinical diagnostic potential of Raman spectroscopy for gliomas. It has been shown that the optical properties of in vitro tissue are strongly dependent upon sample preparation. The investigation of the effects of time latency, paraformalin tissue fixation, and tissue perfusion with carbogen-bubbled cortical transport solution on their respective Raman spectra of brain tissue and tumors will be discussed, as well as their implications on the study of neurological tissue. The studies are conducted with in situ tissue samples from scid mice and 785 nm pulsed alexandrite laser excitation. Results illustrating positive qualitative and quantitative variations between Raman spectra of normal and malignant brain tissue will be presented.

Citation Download Citation

Candace E. Clary, Alex Yu. Dergachev, Sergey B. Mirov, and G. Yancey Gillespie "Raman spectroscopy for in situ- evaluation of high-grade malignant gliomas induced in SCID mice", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273517

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