Rapid multiexcitation spectrofluorimeter for in-vivo tissue diagnosis

Renato Amaro Zangaro; Landulfo Silveira Jr.; Marcos T. T. Pacheco; Ramasamy Manoharan; Irving Itzkan; Ramachandra R. Dasari; Michael S. Feld

doi:10.1117/12.273536

7 May 1997 Rapid multiexcitation spectrofluorimeter for in-vivo tissue diagnosis

Renato Amaro Zangaro, Landulfo Silveira Jr., Marcos T. T. Pacheco, Ramasamy Manoharan, Irving Itzkan, Ramachandra R. Dasari, Michael S. Feld

Author Affiliations +

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273536
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We have designed, fabricated and tested a compact excitation-emission spectrofluorimeter with optical fiber light delivery and collection for use in rapid analysis of tissues in a clinical setting. This system provides ten different excitation wavelengths, permitting collection of all ten emission spectra from biological tissues in approximately 500 ms. It uses a nitrogen laser which pumps a sequence of dyes placed in cuvettes on a rotating wheel. All dye cuvettes utilize a common optical resonant cavity, which simplifies the coupling of the different excitation wavelengths into the delivery fiber of an optical fiber probe. A filter wheel containing one long-pass filter for each excitation wavelength is synchronized with the dye wheel for compactness and ease of control. A white light excitation source permits acquisition of the tissue diffuse reflectance spectrum on each cycle. The fiber optic probe is composed of seven optical fibers arranged at the distal tip in the form of a central delivery fiber surrounded by six collection fibers, with an optical shield. Return fluorescence and reflected light are dispersed by a small spectrograph and detected by a photodiode array detector. The system can collect a single shot spectrum from biological tissue with a signal-to-noise ratio in excess of 50:1. This unique design for providing rapidly variable excitation wavelengths facilitates the collection of multi- excitation fluorescence spectra for the diagnosis of diseases in vivo.

Citation Download Citation

Renato Amaro Zangaro, Landulfo Silveira Jr., Marcos T. T. Pacheco, Ramasamy Manoharan, Irving Itzkan, Ramachandra R. Dasari, and Michael S. Feld "Rapid multiexcitation spectrofluorimeter for in-vivo tissue diagnosis", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273536

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Tissues

Optical fibers

Tissue optics

Dye lasers

Nitrogen lasers

Optical filters

Show All Keywords

Keywords/Phrases

Search In:

Publication Years