Laser SERs spectrum of GLP in Ag sol

PeiDi Bao; Lang Bao; TianQuan Huang; XinMing Liu

doi:10.1117/12.317869

7 August 1998 Laser SERs spectrum of GLP in Ag sol

PeiDi Bao, Lang Bao, TianQuan Huang, XinMing Liu

Proceedings Volume 3548, Biomedical Optics and Lasers: Diagnostics and Treatment; (1998) https://doi.org/10.1117/12.317869
Event: Photonics China '98, 1998, Beijing, China

Abstract

We have obtained and investigated the laser Surface-Enhanced Raman Scattering (SERS) spectrum of Leptospiral Glycolipoproteim (GLP). The SERS spectrum of GLP offers the structural information of the molecules and provides the evidence that the functionality of the molecules are preserved on the Ag surface. The SERS spectrum and its Ordinary Raman Scattering (ORS) spectrum of GLP show their correlations. In the ORS spectrum, the intense AmI at 1651 cm^-1 and weak AmIII at 1283 cm^-1 show that GLP has a predominantly (alpha) -helix secondary structure. The Raman line at 542 cm^-1 indicates that the molecules of GLP has trans-gauch-trans configuration of the C-C-S-S-C-C linkage. In the SERS spectrum, the low-frequency band at 239 cm^-1 is attributed to the metal-molecule vibration, indicating chemisorption of the molecules of GLP on Ag surface. The bands belong to (delta) (COO^-) at 620, 683 cm^-1, the band belongs to (upsilon) _s(C-COO^-) at 921 cm^-1, belongs to (upsilon) (C-N), (upsilon) _s(COO^-) and (delta) (NH₂) at 1158, 1366 and 1648 cm^-1. It suggests that the molecules of GLP were adsorbed on Ag surface through both carboxylate groups and amino groups. The Raman enhancement of GLP has a short-range mechanism. The laser SERS spectrum enhancement factor of GLP is about 3 X 10².

Citation Download Citation

PeiDi Bao, Lang Bao, TianQuan Huang, and XinMing Liu "Laser SERs spectrum of GLP in Ag sol", Proc. SPIE 3548, Biomedical Optics and Lasers: Diagnostics and Treatment, (7 August 1998); https://doi.org/10.1117/12.317869

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