High-speed separation using miniaturized slab gel and high-spatial-resolution detection by a thermal lens microscope

Tamao Odake; Jinjian Zheng; Takehiko Kitamori; Tsuguo Sawada

doi:10.1117/12.335793

4 January 1999 High-speed separation using miniaturized slab gel and high-spatial-resolution detection by a thermal lens microscope

Tamao Odake, Jinjian Zheng, Takehiko Kitamori, Tsuguo Sawada

Author Affiliations +

Proceedings Volume 3565, Thermal Therapy, Laser Welding, and Tissue Interaction; (1999) https://doi.org/10.1117/12.335793
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

Slab gel electrophoresis is a major tool for size-separation of DNA fragments and proteins. However, conventional slab gel electrophoresis uses a large gel to obtain sufficient separation and it takes several hours to separate, because conventional detectors using radio isotopes and fluorescence are affected by the strongly light-scattering gel and do not have enough spatial resolution to be applied to a miniaturized gel. Thermal lens microscope (TLM), which we have developed, has spatial resolution of micrometer level, and is not easily affected by the scattered light and can maintain high spatial resolution. We demonstrate here high- speed separation using a miniaturized gel and successive high spatial resolution detection using TLM. Using a miniaturized gel one-tenth of conventional gel in size, DNA fragments (100 base pair ladder sample) were separated in 5 to 15 minutes, which was about one order of magnitude faster than using a conventional gel, and successfully detected by TLM.

Citation Download Citation

Tamao Odake, Jinjian Zheng, Takehiko Kitamori, and Tsuguo Sawada "High-speed separation using miniaturized slab gel and high-spatial-resolution detection by a thermal lens microscope", Proc. SPIE 3565, Thermal Therapy, Laser Welding, and Tissue Interaction, (4 January 1999); https://doi.org/10.1117/12.335793

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