Novel approach to multichannel SPR sensing

Jiri Homola; Hongbo B. Lu; Garet G. Nenninger; Sinclair S. Yee; Charles T. Campbell

doi:10.1117/12.370287

18 November 1999 Novel approach to multichannel SPR sensing

Jiri Homola, Hongbo B. Lu, Garet G. Nenninger, Sinclair S. Yee, Charles T. Campbell

Proceedings Volume 3857, Chemical Microsensors and Applications II; (1999) https://doi.org/10.1117/12.370287
Event: Photonics East '99, 1999, Boston, MA, United States

Abstract

A novel approach to multichannel SPR sensing based on encoding information from separate sensing channels into a single optical spectrum is presented. A dual channel SPR sensor using this approach is demonstrated. Attention is given to exploitation of the dual-channel SPR sensor for compensation for background interference and non-specific adsorption of the biomolecules to the surface of the SPR biosensor. Experimental results indicate that background refractive index changes were compensated with accuracy better than 8 X 10^-5 RIU (refractive index unit); the effect of a temperature change of 3.6 K was reduced by a factor of 13 by the dual-channel sensor. SPR biosensor-based detection of monoclonal anti-dinitrophenyl antibody (a-DNP) with compensation for non-specific adsorption is demonstrated.

Citation Download Citation

Jiri Homola, Hongbo B. Lu, Garet G. Nenninger, Sinclair S. Yee, and Charles T. Campbell "Novel approach to multichannel SPR sensing", Proc. SPIE 3857, Chemical Microsensors and Applications II, (18 November 1999); https://doi.org/10.1117/12.370287

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