Laser micromachined isoelectric focusing devices on polymer substrate for electrospray mass spectrometry

Yuehe Lin; Jenny Wen; Xiang Fan; Dean W. Matson; Richard D. Smith

doi:10.1117/12.359351

19 August 1999 Laser micromachined isoelectric focusing devices on polymer substrate for electrospray mass spectrometry

Yuehe Lin, Jenny Wen, Xiang Fan, Dean W. Matson, Richard D. Smith

Proceedings Volume 3877, Microfluidic Devices and Systems II; (1999) https://doi.org/10.1117/12.359351
Event: Symposium on Micromachining and Microfabrication, 1999, Santa Clara, CA, United States

Abstract

A microfabricated device for isoelectric focusing (IEF) incorporating an optimized electrospray ionization (ESI) tip was constructed on polycarbonate plates using a laser micromachining technique. The separation channels on an IEF chip were 16 cm long, 50 micrometers wide and 30 micrometers deep. Electrical potentials used for IEF focusing and electrospray were applied through platinum electrodes placed in the buffer reservoirs, and which were isolated from the separation channel by molecular porous membranes. On-line ESI produced directly from a sharp `tip' on the microchip was evaluated. The results indicate that this design can produce a stable electrospray that is further improved and made more flexible with the assistance of sheath gas and sheath liquid. Error analysis of the spectral data shows that the standard deviation in signal intensity for an analyte peak was less than approximately 5% over 3 hours. The production of stable electrosprays directly from microchip IEF devices represents a step towards easily- fabricated microanalytical devices. IEF separations of protein mixtures were demonstrated for uncoated polycarbonate microchips. On-line IEF/ESI-MS was demonstrated using the microfabricated chip with an ion-trap ESI mass spectrometer for characterization of protein mixtures.

Citation Download Citation

Yuehe Lin, Jenny Wen, Xiang Fan, Dean W. Matson, and Richard D. Smith "Laser micromachined isoelectric focusing devices on polymer substrate for electrospray mass spectrometry", Proc. SPIE 3877, Microfluidic Devices and Systems II, (19 August 1999); https://doi.org/10.1117/12.359351

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