Hyperoxygenation enhances photodynamic therapy tumor cure

Fred W. Hetzel; Abdus Shakil; Jill Beckers; Qun Chen

doi:10.1117/12.424435

9 April 2001 Hyperoxygenation enhances photodynamic therapy tumor cure

Fred W. Hetzel, Abdus Shakil, Jill Beckers, Qun Chen

Proceedings Volume 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X; (2001) https://doi.org/10.1117/12.424435
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Tumor hypoxia, either pre-existing or as a result of oxygen bleaching during PDT light irradiation, can significantly reduce the effectiveness of a PDT treatment. To overcome the effect oftumor hypoxia, we propose using supplemental hyperoxygenation during a PDT treatment. The mechanism for the tumor cure enhancement ofthe combined therapy is investigated in the study by using an in vivo/in vitro assay. Tumors treated with PDT/hyperoxygenation are harvested at various times after the light irradiation and cultured in vitro for colony formation, thus separating the direct cell killing by PDT and secondary vascular effect. The results show that, when combined with hyper-oxygenation, the cell survival (colony formation) rate immediately after a PDT treatment is significantly improved over that treated without hyper-oxygenation, suggesting an enhanced direct cell killing. Cell survival rates were unchanged for the first 4 hours after a PDT treatment, but decreased significantly at 1 8 hours, suggesting that secondary vascular damage did take effect, but not until a period of at least 4 hours after light irradiation. These results further confirm our earlier observation that when a PDT treatment is combined with hyper-oxygenation, it can be more effective in controlling hypoxic tumors.

Citation Download Citation

Fred W. Hetzel, Abdus Shakil, Jill Beckers, and Qun Chen "Hyperoxygenation enhances photodynamic therapy tumor cure", Proc. SPIE 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, (9 April 2001); https://doi.org/10.1117/12.424435

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