Novel high-sensitivity fluorescence polarization reader

Clifford C. Hoyt; Richard M. Levenson; Peter Banks

doi:10.1117/12.426755

22 May 2001 Novel high-sensitivity fluorescence polarization reader

Clifford C. Hoyt, Richard M. Levenson, Peter Banks

Proceedings Volume 4255, Clinical Diagnostic Systems; (2001) https://doi.org/10.1117/12.426755
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

We have developed a new fluorescence polarization (FP) reader suitable for high-throughput screening (HST) and ultra-HTS whose assay-performance and sample-throughput are both considerably improved over present state-of-the-art instrumentation. The Symmetry^TM reader possesses a number of features that differ from conventional HTS FP readers. These include: laser-based excitation, liquid crystal polarization optics that rapidly and accurately measure polarization states; and CCD detectors to capture emission from multiple wells. We show that the performance in assays relevant to the drug discovery process, such as G- protein coupled receptor-based assays, is significantly enhanced due to a dramatic improvement in precision. Furthermore, the CCD-detection system used can substantially improve sample throughput compared to sequential readers while maintaining high performance.

Citation Download Citation

Clifford C. Hoyt, Richard M. Levenson, and Peter Banks "Novel high-sensitivity fluorescence polarization reader", Proc. SPIE 4255, Clinical Diagnostic Systems, (22 May 2001); https://doi.org/10.1117/12.426755

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