Generation and propagation of fluorescence light from fluorophores experiencing multiexponential decay kinetics in multiply scattering media

Eddy Kuwana; Eva Marie Sevick-Muraca

doi:10.1117/12.429338

13 June 2001 Generation and propagation of fluorescence light from fluorophores experiencing multiexponential decay kinetics in multiply scattering media

Eddy Kuwana, Eva Marie Sevick-Muraca

Author Affiliations +

Proceedings Volume 4263, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring; (2001) https://doi.org/10.1117/12.429338
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

The generation and propagation of fluorescence light through tissue offers the potential for biomedical diagnostics and clinical biosensing. Arising from an exogenous fluorescent dye injected as a contrast agent or immobilized in a polymer implant, the fluorescent decay kinetics can be sensitive to the biochemical environment of the tissue, providing quantitative in vivo information of the confined tissue site. To date, all studies of fluorescence in scattering media have been confined to dyes with single-exponential decay kinetics, yet most of sensing fluorophores exhibit multi-exponential decay. The goal of the study was to develop a time-dependent model describing the generation and propagation of fluorescent light emitted from dyes exhibiting multi-exponential decay kinetics. To experimentally investigate multi-exponential decays, we employed two dyes, 3,3-diethylthiatricarbocyanine iodide (DTTCI) and Indocynanine Green (ICG), which exhibit distinctly different lifetimes. The experimental study was divided into two parts. In the first part, fluorescence phase-modulation measurements were made as a function of modulation frequency at various concentration ratios of the two dyes in dilute nonscattering solutions. In the second part, frequency measurements of phase-modulation were made at similar concentration ratios as the first part, but in presence of scattering (2% intralipid). The multi-exponential decay parameters extracted from the phase-modulation data in the first part of the study were used to predict the phase-shift and amplitude-attenuation values in the presence of scattering. Using a diffusion model, the predictions were then compared to the second part of the experimental study.

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Eddy Kuwana and Eva Marie Sevick-Muraca "Generation and propagation of fluorescence light from fluorophores experiencing multiexponential decay kinetics in multiply scattering media", Proc. SPIE 4263, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring, (13 June 2001); https://doi.org/10.1117/12.429338

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