Novel fiber optic glucose biosensor based on fluorescence quenching

Desheng Jiang; Er Liu; Jun Huang

doi:10.1117/12.481974

9 September 2002 Novel fiber optic glucose biosensor based on fluorescence quenching

Desheng Jiang, Er Liu, Jun Huang

Proceedings Volume 4920, Advanced Sensor Systems and Applications; (2002) https://doi.org/10.1117/12.481974
Event: Photonics Asia, 2002, Shanghai, China

Abstract

A new fiber optic glucose biosensor based on oxygen fluorescence quenching using lock-in technology has been presented. Ruthenium(II) complex, Ru(bpy)3Cl2, were used as the fluorescence indicator and cellulose acetate(CA) were used as the matrix membrane to immobilize the indicator and GOD, and the optimal conditions for the preparation of CA membrane has been studied. For the immobilization of GOD to CA membrane, albumin of bovine serum(BSA) and glutaraldehyde(GA) were used through covalence—cross bonding process. The relationship between the concentration of glucose and the phase delay??? has been studied, the results show that ??? has good relationship within the range of 50mg/dl—500mg/dl and the response time is less than 30 sec. Several factors to influence the sensor such as pH of the medium, the additional membrane layers, the properties of CA membrane have also been studied. The results indicate that its best pH range is between pH6.0 and 7.0, and the thickness of the additional membrane layers on the sensor head will influence the response time greatly, that is, the response time is controlled by the speed of dispersion of the dissolved oxygen into the fluorescence dye layer.

Citation Download Citation

Desheng Jiang, Er Liu, and Jun Huang "Novel fiber optic glucose biosensor based on fluorescence quenching", Proc. SPIE 4920, Advanced Sensor Systems and Applications, (9 September 2002); https://doi.org/10.1117/12.481974

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available