The photoinduced conformational change in macromolecules

Y. Barabash; V. Kharkyanen; A. Kudrjavtsev; M. A. Zabolotny; Nickolai I. Sokolov

doi:10.1117/12.569840

20 July 2004 The photoinduced conformational change in macromolecules

Y. Barabash, V. Kharkyanen, A. Kudrjavtsev, M. A. Zabolotny, Nickolai I. Sokolov

Proceedings Volume 5507, XVI International Conference on Spectroscopy of Molecules and Crystals; (2004) https://doi.org/10.1117/12.569840
Event: XVI International Conference on Spectroscopy of Molecules and Crystals, 2003, Sevastopol, Ukraine

Abstract

This paper concerns with our measurements of reaction center (RC) volume changes in the course of photoactivation using method of holographic interferometry. As a principal value, we studed change of a solution refraction index (▵n) influenced by the sample volume expansion due to temperature changes (▵n_T), light absorption (▵n_abs) and changes in the volume of photoactive molecules (▵n_V). Using the scheme of temperature compensation we could reduce the task of the case ▵n_T<<▵n_abs, ▵n_V Our estimation for the case of a protein solution yields in relative changes in the molecular volume within 10^-3 ÷ 10^-2.

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Y. Barabash, V. Kharkyanen, A. Kudrjavtsev, M. A. Zabolotny, and Nickolai I. Sokolov "The photoinduced conformational change in macromolecules", Proc. SPIE 5507, XVI International Conference on Spectroscopy of Molecules and Crystals, (20 July 2004); https://doi.org/10.1117/12.569840

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KEYWORDS

Refractive index

Absorption

Molecules

Macromolecules

Sodium

Proteins

Temperature metrology

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