Immobilized DNA biosensor based on evanescent wave long-period fiber gratings

Jian Yang; Chang-Qing Xu; Razvan Nutiu; Yingfu Li

doi:10.1117/12.567502

9 December 2004 Immobilized DNA biosensor based on evanescent wave long-period fiber gratings

Jian Yang, Chang-Qing Xu, Razvan Nutiu, Yingfu Li

Proceedings Volume 5578, Photonics North 2004: Photonic Applications in Astronomy, Biomedicine, Imaging, Materials Processing, and Education; (2004) https://doi.org/10.1117/12.567502
Event: Photonics North, 2004, Ottawa, Ontario, Canada

Abstract

A DNA aptamer sensor using a long-period grating (LPG) is proposed for the sensitive detection of the interaction between the DNA aptamer and its target. The DNA aptamer can be immobilized on the surface of the LPG, and the detection of the specific interaction between the DNA aptamer and its target is monitored optically. This sensor operates on the evanescent field interacting with the DNA aptamer immobilized over the grating region. The reaction between the DNA aptamer and its target alter the refractive index and thus change the LPG transmission spectrum, which can be monitored in real time. This technology eliminates the labeling of the DNA aptamer. In this paper, for the first time, the dependence of the LPG transmission spectrum on the characteristic of the LPG and the property of the immobilized DNA aptamer are studied both theoretically and experimentally. Experimental demonstration of the immobilization of DNA aptamer on LPG and its real-time effect on the transmission spectrum are reported.

Citation Download Citation

Jian Yang, Chang-Qing Xu, Razvan Nutiu, and Yingfu Li "Immobilized DNA biosensor based on evanescent wave long-period fiber gratings", Proc. SPIE 5578, Photonics North 2004: Photonic Applications in Astronomy, Biomedicine, Imaging, Materials Processing, and Education, (9 December 2004); https://doi.org/10.1117/12.567502

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