Novel confocal microarray scanner for fluorescence detection

Liqiang Wang; Xuxiang Ni; Zukang Lu

doi:10.1117/12.573428

18 January 2005 Novel confocal microarray scanner for fluorescence detection

Liqiang Wang, Xuxiang Ni, Zukang Lu

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.573428
Event: Photonics Asia, 2004, Beijing, China

Abstract

A novel confocal microarray scanner was introduced, which employed a 532nm laser and a 635nm laser to excite Cy3 and Cy5 fluorophores respectively. The fluorescent signal was detected using a photomultiplier tube (PMT) sequentially. One dimension scanning of the microarray slide was performed by a telecentric objective with a moving coil optical scanner; the other dimension was scanned by a stepping motor driving the precise guidance. Experimental results show that scanning resolution of the presented microarray scanner can reach 5 microns, its dynamic range is near 4 orders of magnitude and the limit of detection is 1.12 about fluors per square micron. The cross-talk error is eliminated almost completely by its sequential scanning mode.

Citation Download Citation

Liqiang Wang, Xuxiang Ni, and Zukang Lu "Novel confocal microarray scanner for fluorescence detection", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.573428

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