The Reconstruction of Three-Dimensional Morphological and Electrical Paraneters from Two-Dimensional Sections of Neurones

A. D. Brawn; H. V. Wheal

doi:10.1117/12.956337

7 July 1986 The Reconstruction of Three-Dimensional Morphological and Electrical Paraneters from Two-Dimensional Sections of Neurones

A. D. Brawn, H. V. Wheal

Author Affiliations +

Proceedings Volume 0602, Biostereometrics '85; (1986) https://doi.org/10.1117/12.956337
Event: 1985 International Technical Symposium/Europe, 1985, Cannes, France

Abstract

A system is described which can be used to create a three-dimensional model of a neurone from the central nervous system. This model can then be used to obtain quantitative data on the physical and electrical pro, perties of the neurone. Living neurones are either raised in culture, or taken from in vitro preparations of brain tissue and optically sectioned. These two-dimensional sections are digitised, and input to a 68008-based microcomputer. The system reconstructs the three-dimensional structure of the neurone, both geanetrically and electrically. The user can a) View the structure fran any point at any angle b) "Move through" the structure along any given vector c) Nave through" the structure following a neurone process d) Fire the neurone at any point, and "watch" the action potentials propagate e) Vary the parameters of the electrical model of a process element. The system is targeted to a research programme on epilepsy, which makes frequent use of both geometric and electrical neurone modelling. Current techniques which may involve crude histology and two-dimensional drawings have considerable short camings.

Citation Download Citation

A. D. Brawn and H. V. Wheal "The Reconstruction of Three-Dimensional Morphological and Electrical Paraneters from Two-Dimensional Sections of Neurones", Proc. SPIE 0602, Biostereometrics '85, (7 July 1986); https://doi.org/10.1117/12.956337

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