Detection of ultrastructural changes in genetically altered and exercised skeletal muscle using PS-OCT

James J. Pasquesi; Simon C Schlachter; Marni D. Boppart; Eric Chaney; Stephen J. Kaufman; Stephen A. Boppart

doi:10.1117/12.646584

20 February 2006 Detection of ultrastructural changes in genetically altered and exercised skeletal muscle using PS-OCT

James J. Pasquesi, Simon C Schlachter, Marni D. Boppart, Eric Chaney, Stephen J. Kaufman, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 6079, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X; 607926 (2006) https://doi.org/10.1117/12.646584
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Birefringence of skeletal muscle has been associated with the ultrastructure of individual sarcomeres, specifically the arrangement of A-bands corresponding to the thick myosin filaments. Murine skeletal muscle (gastrocnemius) was imaged with a fiber-based PS-OCT imaging system to determine the level of birefringence present in the tissue under various conditions. In addition to muscle controls from wild-type mice, muscle from abnormal mice included: genetically-modified (mdx) mice which model human muscular dystrophy, transgenic mice exhibiting an overexpression of integrin (α7β1), and transgenic integrin (α7β1)knockout mice. Comparisons were also made between rested and exercised muscles to determine the effects of exercise on muscle birefringence for each of these normal and abnormal conditions. The PS-OCT images revealed that the presence of birefringence was similar in the rested muscle with dystrophy-like features (i.e., lacking the structural protein dystrophin - mdx) and in the integrin (α7β1)knockout muscle when compared to the normal (wild-type) control. However, exercising these abnormal muscle tissues drastically reduced the presence of birefringence detected by the PS-OCT system. The muscle exhibiting an overexpression of integrin (α7β1) remained heavily birefringent before and after exercise, similar to the normal (wild-type) muscle. These results suggest that there is a distinct relationship between the degree of birefringence detected using PS-OCT and the sarcomeric ultrastructure present within skeletal muscle.

Citation Download Citation

James J. Pasquesi, Simon C Schlachter, Marni D. Boppart, Eric Chaney, Stephen J. Kaufman, and Stephen A. Boppart "Detection of ultrastructural changes in genetically altered and exercised skeletal muscle using PS-OCT", Proc. SPIE 6079, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X, 607926 (20 February 2006); https://doi.org/10.1117/12.646584

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE