Comparison of ALA and Photofrin in two rat glioma models

Sarah A. Ziegler; Casey Hall; Cherisse Loucks; Steen J. Madsen; Stephen W. Carper

doi:10.1117/12.716527

23 March 2007 Comparison of ALA and Photofrin in two rat glioma models

Sarah A. Ziegler, Casey Hall, Cherisse Loucks, Steen J. Madsen, Stephen W. Carper

Proceedings Volume 6424, Photonic Therapeutics and Diagnostics III; 64242E (2007) https://doi.org/10.1117/12.716527
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Photodynamic therapy (PDT) shows good promise as an adjuvant therapy for the treatment of gliomas. PDT requires the use of a photosensitizer but there have been few studies in which they have been directly compared. Two rat brain glioma cell lines, BT4C and F98 were used to study the effects of Photofrin (PF) and aminolevulinic acid (ALA) as photosensitizers. Both compounds were able to sensitize cells to red light (635nm) however, PF treated (2.5 &mgr;g/ml) cells displayed more cell death than ALA treated (1 mM) cells. ALA-PDT treated cells did not have a block in the cell cycle while PF-PDT treated cells had a fluence dependent G1 block 24 hrs after treatment. The BT4C cell line also displayed a G2 cell cycle block. Both photosensitizers were able to induce apoptosis as measured by flow cytometry but only PF-PDT was able to induce necrosis at the fluence rates investigated (1-20 J/cm²). These differences in cellular responses to ALA and

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Sarah A. Ziegler, Casey Hall, Cherisse Loucks, Steen J. Madsen, and Stephen W. Carper "Comparison of ALA and Photofrin in two rat glioma models", Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64242E (23 March 2007); https://doi.org/10.1117/12.716527

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