Use of optical pharmacokinetics systems (OPS) for non-invasive measurement of Phthalocyanine 4 (Pc 4) concentrations in mice bearing MDA-MB-231 xenografts

Lihua Bai; Erin Joseph; Nancy L. Olenick; John M. Mulvihill; Denise K. Feyes; Julie L. Eiseman

doi:10.1117/12.778081

26 October 2007 Use of optical pharmacokinetics systems (OPS) for non-invasive measurement of Phthalocyanine 4 (Pc 4) concentrations in mice bearing MDA-MB-231 xenografts

Lihua Bai, Erin Joseph, Nancy L. Olenick, John M. Mulvihill, Denise K. Feyes, Julie L. Eiseman

Author Affiliations +

Proceedings Volume 6796, Photonics North 2007; 67960L (2007) https://doi.org/10.1117/12.778081
Event: Photonics North 2007, 2007, Ottawa, Canada

Abstract

Objective: Pc 4, a phthalocyanine photosensitizer in Phase I photodynamic therapy (PDT) trials, requires laser activation near 672 nm. For effective PDT, photosensitizer must be present in the target tissues. OPS uses elastic scattering spectroscopy to measure Pc 4 optical absorption non-invasively, and that absorbance can be converted to concentration using Pc 4 standard curves in 1% Intralipid®. In this study, we used OPS to evaluate Pc 4 optical absorption with time in subcutaneous tumor (with or without laser activation) and in contralateral skin. Tumor response was also evaluated after Pc 4-PDT. Conclusions: Both Pc 4 and hemoglobin optical absorption could be monitored by OPS. The decrease of Pc 4 absorption after PDT and the appearance of d-hbg indicated that alterations occurred in the tumor following Pc 4-PDT. The increase in d-hbg suggests that oxygen was not replaced completely, possibly due to circulation damage in tumor.

Citation Download Citation

Lihua Bai, Erin Joseph, Nancy L. Olenick, John M. Mulvihill, Denise K. Feyes, and Julie L. Eiseman "Use of optical pharmacokinetics systems (OPS) for non-invasive measurement of Phthalocyanine 4 (Pc 4) concentrations in mice bearing MDA-MB-231 xenografts", Proc. SPIE 6796, Photonics North 2007, 67960L (26 October 2007); https://doi.org/10.1117/12.778081

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