Determine scattering coefficient and anisotropy of scattering of tissue phantoms using reflectance-mode confocal microscopy

Ravikant Samatham; Steven L. Jacques

doi:10.1117/12.809684

24 February 2009 Determine scattering coefficient and anisotropy of scattering of tissue phantoms using reflectance-mode confocal microscopy

Ravikant Samatham, Steven L. Jacques

Author Affiliations +

Proceedings Volume 7187, Biomedical Applications of Light Scattering III; 718711 (2009) https://doi.org/10.1117/12.809684
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Different techniques have been developed to determine the optical properties of turbid media, which include collimated transmission, diffuse reflectance, adding-doubling and goniometry. While goniometry can be used to determine the anisotropy of scattering (g), other techniques are used to measure the absorption coefficient and reduced scattering coefficient (μ_s(1-g)). But separating scattering coefficient (μ_s) and anisotropy of scattering from reduced scattering coefficient has been tricky. We developed an algorithm to determine anisotropy of scattering from the depth dependent decay of reflectance-mode confocal scanning laser microscopy (rCSLM) data. This report presents the testing of the algorithm on tissue phantoms with different anisotropies (g = 0.127 to 0.868, at 488nm wavelength). Tissue phantoms were made from polystyrene microspheres (6 sizes 0.1-0.36 μm dia.) dispersed in both aqueous solutions. Three dimensional images were captured. The rCSLM-signal followed an exponential decay as a function of depth of the focal volume, R(z) = ρexp(-μz) where ρ (dimensionless, ρ=1 for a mirror) is the local reflectivity and μ [cm^-1] is the exponential decay constant. The theory was developed to uniquely map the experimentally determined μ and ρ into the optical scattering properties μ_s and g. The values of μ_s and g depend on the composition and microstructure of tissues, and allow characterization of a tissue.

Citation Download Citation

Ravikant Samatham and Steven L. Jacques "Determine scattering coefficient and anisotropy of scattering of tissue phantoms using reflectance-mode confocal microscopy", Proc. SPIE 7187, Biomedical Applications of Light Scattering III, 718711 (24 February 2009); https://doi.org/10.1117/12.809684

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available