Time-resolved FRET for single-nucleotide polymorphism genotyping

Alessandra Andreoni; Luca Nardo; Maria Bondani

doi:10.1117/12.818461

29 April 2009 Time-resolved FRET for single-nucleotide polymorphism genotyping

Alessandra Andreoni, Luca Nardo, Maria Bondani

Proceedings Volume 7320, Advanced Photon Counting Techniques III; 732014 (2009) https://doi.org/10.1117/12.818461
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

By tens-of-picosecond resolved fluorescence detection (TCSPC, time-correlated single-photon counting) we study Förster resonance energy transfer between a donor and a black-hole-quencher acceptor bound at the 5'- and 3'-positions of a synthetic DNA oligonucleotide. This dual labelled oligonucleotide is annealed with either the complementary sequence or with sequences that mimic single-nucleotide polymorphic gene sequences: they differ in one nucleotide at positions near either the ends or the center of the oligonucleotide. We find donor fluorescence decay times whose values are definitely distinct and discuss the feasibility of single nucleotide polymorphism genotyping by this method.

Citation Download Citation

Alessandra Andreoni, Luca Nardo, and Maria Bondani "Time-resolved FRET for single-nucleotide polymorphism genotyping", Proc. SPIE 7320, Advanced Photon Counting Techniques III, 732014 (29 April 2009); https://doi.org/10.1117/12.818461

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