A simple enzyme based biosensor on flexible plastic substrate

Senaka K. Kanakamedala; Haidar T. Alshakhouri; Mangilal Agarwal; Ji Fang; Mark A. DeCoster

doi:10.1117/12.860587

24 August 2010 A simple enzyme based biosensor on flexible plastic substrate

Senaka K. Kanakamedala, Haidar T. Alshakhouri, Mangilal Agarwal, Ji Fang, Mark A. DeCoster

Proceedings Volume 7759, Biosensing III; 77590F (2010) https://doi.org/10.1117/12.860587
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

An enzyme based biosensor was fabricated by employing a simple, inexpensive and rapid xurography fabrication process. The electrodes and channel were made from the conducting polymer poly(3,4-ethyelenedioxythiphene) poly(styrene sulfonate) (PEDOT:PSS). PEDOT:PSS was selectively deposited using a polyimide tape mask. The tape mask was peeled off from the substrate after annealing the polymer in vacuum. Polymer wells of defined dimensions were made and were attached to the device to accommodate the solutions. This sensor utilizes the change in current as a parameter to measure different analyte concentrations. Initial experiments were done by using the sensor for glucose detection. The sensor is able to detect the glucose concentrations approximately from 1 μM to 10 mM range covering glucose in human saliva (8-210 μM). The glucose oxidase activity was independently measured using colorimetric method and the results indicate that the sensor retains the enzyme activity and can be used as a biosensor to detect various analytes. The analyte of interest can be measured by preloading the corresponding enzyme into the wells.

Citation Download Citation

Senaka K. Kanakamedala, Haidar T. Alshakhouri, Mangilal Agarwal, Ji Fang, and Mark A. DeCoster "A simple enzyme based biosensor on flexible plastic substrate", Proc. SPIE 7759, Biosensing III, 77590F (24 August 2010); https://doi.org/10.1117/12.860587

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available