A novel method for eliminating autofluorescence of small animals in fluorescence molecular imaging

Zhenwen Xue; Jie Tian; Dong Han; Xibo Ma

doi:10.1117/12.878083

9 March 2011 A novel method for eliminating autofluorescence of small animals in fluorescence molecular imaging

Zhenwen Xue, Jie Tian, Dong Han, Xibo Ma

Proceedings Volume 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging; 79651D (2011) https://doi.org/10.1117/12.878083
Event: SPIE Medical Imaging, 2011, Lake Buena Vista (Orlando), Florida, United States

Abstract

As a newly emerged optical imaging method, fluorescence molecular imaging technique has been receiving increasing attention for its ability of non-invasive visualization of the cellular and molecular activities. However, as a kind of background noise, autofluorescence is a major disturbing factor in fluorescence molecular imaging. In this paper, we proposed a novel method to eliminate autofluorescence of small animals. The method is based on the fact that most autofluorescent signal has a broad excitation and emission spectrum, whereas specific fluorescent probe has a narrow one. First, two fluorescent images are obtained at two different excitation wavelengths. Then we divide the two obtained fluorescent images into blocks with the size of 8×8 pixel. The two blocks from the same position of the two different images respectively constitute a block pair. The ratio of one block's summation of total pixel value to that of ther other block belonging to the same block pair is calculated. After that, we classify all block pairs into fluorescent and nonfluorescent ones by ratio. The former are considered to be actual fluorescent regions. In next step, we adopt an adaptive cluster analysis method to classify all fluorescent block pairs into multiple interest regions. A general centroid algorithm is then applied to locate the center of each interest regions. We recover the fluorescent interest regions using flood filling algorithm. Finally, we choose a GFP-transfected tumor mouse model and a GFP-transplanted mouse skin model to validate our algorithm.

Citation Download Citation

Zhenwen Xue, Jie Tian, Dong Han, and Xibo Ma "A novel method for eliminating autofluorescence of small animals in fluorescence molecular imaging", Proc. SPIE 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging, 79651D (9 March 2011); https://doi.org/10.1117/12.878083

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available