In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation

Nora Bloise; Enrica Saino; Francesca Bragheri; Paolo Minzioni; Ilaria Cristiani; Marcello Imbriani; Livia Visai

doi:10.1117/12.889873

2 June 2011 In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation

Nora Bloise, Enrica Saino, Francesca Bragheri, Paolo Minzioni, Ilaria Cristiani, Marcello Imbriani, Livia Visai

Proceedings Volume 8092, Medical Laser Applications and Laser-Tissue Interactions V; 80920U (2011) https://doi.org/10.1117/12.889873
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

The objective of this study was to examine the in vitro effect of a single or a multiple doses of low-level laser irradiation (LLLI) on proliferation of the human osteosarcoma cell line, SAOS-2. SAOS-2 cells were divided in five groups and exposed to LLLI (659 nm diode laser; 11 mW power output): group I as a control (dark), group II exposed to a single laser dose of 1 J/cm², group III irradiated with a single dose of 3 J/cm², and group IV and V exposed for three consecutive days to 1 or 3 J/cm², respectively. Cellular proliferation was assessed daily up to 7 days of culturing. The obtained results showed an increase in proliferative capacity of SAOS-2 cells during the first 96 h of culturing time in once-irradiated cells, as compared to control cells. Furthermore, a significantly higher proliferation in the group IV and V was detected if compared to a single dose or to control group after 96 h and 7 days. In conclusion, the effect of the single dose on cell proliferation was transitory and repeated irradiations were necessary to observe a strong enhancement of SAOS-2 growth. As a future perspective, we would like to determine the potential of LLLI as a new approach for promoting bone regeneration onto biomaterials.

Citation Download Citation

Nora Bloise, Enrica Saino, Francesca Bragheri, Paolo Minzioni, Ilaria Cristiani, Marcello Imbriani, and Livia Visai "In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation", Proc. SPIE 8092, Medical Laser Applications and Laser-Tissue Interactions V, 80920U (2 June 2011); https://doi.org/10.1117/12.889873

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Laser irradiation

In vitro testing

Semiconductor lasers

Bone

Scanning electron microscopy

Laser therapeutics

Carbon dioxide

Show All Keywords

Keywords/Phrases

Search In:

Publication Years