High-frequency H-PDLC optical chopper for frequency division multiplexing fluorescence confocal microscope system

Yanmeng Jiang; Jihong Zheng; Pingyu Tang; Tingting Wang; Aiqin Huang; Zengjun Zhou; Songlin Zhuang

doi:10.1117/12.897220

7 September 2011 High-frequency H-PDLC optical chopper for frequency division multiplexing fluorescence confocal microscope system

Yanmeng Jiang, Jihong Zheng, Pingyu Tang, Tingting Wang, Aiqin Huang, Zengjun Zhou, Songlin Zhuang

Author Affiliations +

Proceedings Volume 8120, Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications V; 81201O (2011) https://doi.org/10.1117/12.897220
Event: SPIE Photonic Devices + Applications, 2011, San Diego, California, United States

Abstract

The optical chopper array based on Holographic Polymer Dispersed Liquid Crystal (H-PDLC) working at high frequencies, for example 1KHz, 2KHz, and its application in an improved Frequency Division Multiplexed Fluorescence Confocal Microscope (FDMFCM) system are reported in this article. The system is a combination of the confocal microscopy and the frequency division multiplexing technique. Taking advantages of the optical chopper array based on H-PDLC that avoids mechanical movements, the FDMFCM system is able to obtain better Signal-Noise Ratio (SNR), smaller volume, more independent channels and more efficient scanning. What's more, the FDMCFM maintained the high special resolution ability and realized faster temporal resolution than pervious system. Using the proposed device, the FDMFCM system conducts successful parallel detection of rat neural cells. Fluorescence intensity signals from two different points on the specimen, which represent concentration of certain kind of proteins in the sample cells, are achieved. The experimental results show that the proposed H-PDLC optical chopper array has feasibility in FDMFCM system, which owes to its unique characteristics such as fast response, simple fabrication and lower consumption etc. With the development of H-PDLC based devices, there will be prospective in upgrading FDMFCM system's performance in the biomedical area.

Citation Download Citation

Yanmeng Jiang, Jihong Zheng, Pingyu Tang, Tingting Wang, Aiqin Huang, Zengjun Zhou, and Songlin Zhuang "High-frequency H-PDLC optical chopper for frequency division multiplexing fluorescence confocal microscope system", Proc. SPIE 8120, Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications V, 81201O (7 September 2011); https://doi.org/10.1117/12.897220

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Optical choppers

Liquid crystals

Luminescence

Polymers

Confocal microscopy

Optical arrays

Holography

Show All Keywords

Keywords/Phrases

Search In:

Publication Years