Colony image acquisition and genetic segmentation algorithm and colony analyses

W. X. Wang

doi:10.1117/12.913588

24 January 2012 Colony image acquisition and genetic segmentation algorithm and colony analyses

W. X. Wang

Proceedings Volume 8292, Color Imaging XVII: Displaying, Processing, Hardcopy, and Applications; 82921C (2012) https://doi.org/10.1117/12.913588
Event: IS&T/SPIE Electronic Imaging, 2012, Burlingame, California, United States

Abstract

Colony anaysis is used in a large number of engineerings such as food, dairy, beverages, hygiene, environmental monitoring, water, toxicology, sterility testing. In order to reduce laboring and increase analysis acuracy, many researchers and developers have made efforts for image analysis systems. The main problems in the systems are image acquisition, image segmentation and image analysis. In this paper, to acquire colony images with good quality, an illumination box was constructed. In the box, the distances between lights and dishe, camra lens and lights, and camera lens and dishe are adjusted optimally. In image segmentation, It is based on a genetic approach that allow one to consider the segmentation problem as a global optimization,. After image pre-processing and image segmentation, the colony analyses are perfomed. The colony image analysis consists of (1) basic colony parameter measurements; (2) colony size analysis; (3) colony shape analysis; and (4) colony surface measurements. All the above visual colony parameters can be selected and combined together, used to make a new engineeing parameters. The colony analysis can be applied into different applications.

Citation Download Citation

W. X. Wang "Colony image acquisition and genetic segmentation algorithm and colony analyses", Proc. SPIE 8292, Color Imaging XVII: Displaying, Processing, Hardcopy, and Applications, 82921C (24 January 2012); https://doi.org/10.1117/12.913588

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available