Mobile large area confocal scanner for imaging tumor margins: initial testing in the pathology department

Sanjee Abeytunge; Yongbiao Li; Bjorg Larson; Gary Peterson; Ricardo Toledo-Crow; Milind Rajadhyaksha

doi:10.1117/12.2005026

22 March 2013 Mobile large area confocal scanner for imaging tumor margins: initial testing in the pathology department

Sanjee Abeytunge, Yongbiao Li, Bjorg Larson, Gary Peterson, Ricardo Toledo-Crow, Milind Rajadhyaksha

Proceedings Volume 8572, Advanced Biomedical and Clinical Diagnostic Systems XI; 85720U (2013) https://doi.org/10.1117/12.2005026
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Surgical oncology is guided by examining pathology that is prepared during or after surgery. The preparation time for Mohs surgery in skin is 20-45 minutes, for head-and-neck and breast cancer surgery is hours to days. Often this results in incomplete tumor removal such that positive margins remain. However, high resolution images of excised tissue taken within few minutes can provide a way to assess the margins for residual tumor. Current high resolution imaging methods such as confocal microscopy are limited to small fields of view and require assembling a mosaic of images in two dimensions (2D) to cover a large area, which requires long acquisition times and produces artifacts. To overcome this limitation we developed a confocal microscope that scans strips of images with high aspect ratios and stitches the acquired strip-images in one dimension (1D). Our “Strip Scanner” can image a 10 x 10 mm² area of excised tissue with sub-cellular detail in about one minute. The strip scanner was tested on 17 Mohs excisions and the mosaics were read by a Mohs surgeon blinded to the pathology. After this initial trial, we built a mobile strip scanner that can be moved into different surgical settings. A tissue fixture capable of scanning up to 6 x 6 cm² of tissue was also built. Freshly excised breast and head-and-neck tissues were imaged in the pathology lab. The strip-images were registered and displayed simultaneously with image acquisition resulting in large, high-resolution confocal mosaics of fresh surgical tissue in a clinical setting.

Citation Download Citation

Sanjee Abeytunge, Yongbiao Li, Bjorg Larson, Gary Peterson, Ricardo Toledo-Crow, and Milind Rajadhyaksha "Mobile large area confocal scanner for imaging tumor margins: initial testing in the pathology department", Proc. SPIE 8572, Advanced Biomedical and Clinical Diagnostic Systems XI, 85720U (22 March 2013); https://doi.org/10.1117/12.2005026

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KEYWORDS

Tissues

Confocal microscopy

Pathology

Surgery

Tumors

Mohs surgery

Tissue optics

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