High-spectral resolution nonlinear microspectroscopy and imaging of soft condensed and biological media

Feruz Ganikhanov; Shan Yang; Sanjay Adhikari

doi:10.1117/12.2003240

19 March 2013 High-spectral resolution nonlinear microspectroscopy and imaging of soft condensed and biological media

Feruz Ganikhanov, Shan Yang, Sanjay Adhikari

Proceedings Volume 8577, Optical Biopsy XI; 85770Q (2013) https://doi.org/10.1117/12.2003240
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Precise information on dispersion of the nonlinear optical susceptibility of Raman active media is essential in order to get an insight into physics and chemistry of intra- and inter-molecular interactions. We propose and experimentally demonstrate a method that is capable of resolving both real and imaginary parts of third-order nonlinearity (χ⁽³⁾) in the vicinity of Raman resonances. Dispersion of χ⁽³⁾ can be obtained from a medium probed within microscopic volumes with a spectral resolution of better than 0.1 cm^-1 thus making our approach an essential tool in quantitative microscopic characterization of complex biological media. Time-domain CARS transients traced with femtosecond pulses within orders of magnitude in the signal decay can lead to resolution of fine spectral features in χ⁽³⁾ dispersion that can not be reliably detected by frequency-domain Raman based spectroscopy/microscopy techniques, including coherent methods. We will present results of the method’s application in biological cells and tissue. Namely, we accessed a protein line at 1245 cm^-1 in E-coli cell, major DNA and protein lines in red blood cells and triglyceride Raman active peaks in fat tissue.

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Feruz Ganikhanov, Shan Yang, and Sanjay Adhikari "High-spectral resolution nonlinear microspectroscopy and imaging of soft condensed and biological media", Proc. SPIE 8577, Optical Biopsy XI, 85770Q (19 March 2013); https://doi.org/10.1117/12.2003240

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KEYWORDS

Raman spectroscopy

Tissues

Signal detection

Optical parametric oscillators

Microscopy

Femtosecond phenomena

Imaging spectroscopy

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