Probing multiscale mechanics of collagen with optical tweezers

Marjan Shayegan; Naghmeh Rezaei; Norman H. Lam; Tuba Altindal; Andrew Wieczorek; Nancy R. Forde

doi:10.1117/12.2027258

12 September 2013 Probing multiscale mechanics of collagen with optical tweezers

Marjan Shayegan, Naghmeh Rezaei, Norman H. Lam, Tuba Altindal, Andrew Wieczorek, Nancy R. Forde

Proceedings Volume 8810, Optical Trapping and Optical Micromanipulation X; 88101P (2013) https://doi.org/10.1117/12.2027258
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

How the molecular structure of the structural, extracellular matrix protein collagen correlates with its mechanical properties at different hierarchical structural levels is not known. We demonstrate the utility of optical tweezers to probe collagen’s mechanical response throughout its assembly hierarchy, from single molecule force-extension measurements through microrheology measurements on solutions of collagen molecules, collagen fibrillar gels and gelatin. These experiments enable the determination of collagen’s flexibility, mechanics, and timescales and strengths of interaction at different levels of hierarchy, information critical to developing models of how collagen’s physiological function and stability are influenced by its chemical composition. By investigating how the viscoelastic properties of collagen are affected by the presence of telopeptides, protein domains that strongly influence fibril formation, we demonstrate that these play a role in conferring transient elasticity to collagen solutions.

Citation Download Citation

Marjan Shayegan, Naghmeh Rezaei, Norman H. Lam, Tuba Altindal, Andrew Wieczorek, and Nancy R. Forde "Probing multiscale mechanics of collagen with optical tweezers", Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 88101P (12 September 2013); https://doi.org/10.1117/12.2027258

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