Manipulation of yeast cells with DOE-modulated crescent-shaped and optical bottle laser beams

Mikhail A. Rykov; Roman V. Skidanov

doi:10.1117/12.2027015

12 September 2013 Manipulation of yeast cells with DOE-modulated crescent-shaped and optical bottle laser beams

Mikhail A. Rykov, Roman V. Skidanov

Proceedings Volume 8810, Optical Trapping and Optical Micromanipulation X; 88102Z (2013) https://doi.org/10.1117/12.2027015
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

The work is a study of micromanipulation techniques using DOE-modulated laser beams. Two kinds of crescentshaped and optical bottle beam kinds are covered. The numerical model and experimental results on the trapping and manipulating of yeast cells with modulated laser beams are presented. They show that modulated beams are sometimes more efficient than common Gaussian beam: crescent beams are able to achieve better trap stiffness at the price of altered spatial distribution of that stiffness. Optical bottles are beneficial for their ability to hold the trapped object by its edges therefore decreasing amount of energy absorbed by the microbiological sample.

Citation Download Citation

Mikhail A. Rykov and Roman V. Skidanov "Manipulation of yeast cells with DOE-modulated crescent-shaped and optical bottle laser beams", Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 88102Z (12 September 2013); https://doi.org/10.1117/12.2027015

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