Quantitative fiber-optic Raman spectroscopy for tissue Raman measurements

Shiyamala Duraipandian; Mads Bergholt; Wei Zheng; Zhiwei Huang

doi:10.1117/12.2039576

4 March 2014 Quantitative fiber-optic Raman spectroscopy for tissue Raman measurements

Shiyamala Duraipandian, Mads Bergholt, Wei Zheng, Zhiwei Huang

Proceedings Volume 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry; 89390V (2014) https://doi.org/10.1117/12.2039576
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Molecular profiling of tissue using near-infrared (NIR) Raman spectroscopy has shown great promise for in vivo detection and prognostication of cancer. The Raman spectra measured from the tissue generally contain fundamental information about the absolute biomolecular concentrations in tissue and its changes associated with disease transformation. However, producing analogues tissue Raman spectra present a great technical challenge. In this preliminary study, we propose a method to ensure the reproducible tissue Raman measurements and validated with the in vivo Raman spectra (n=150) of inner lip acquired using different laser powers (i.e., 30 and 60 mW). A rapid Raman spectroscopy system coupled with a ball-lens fiber-optic Raman probe was utilized for tissue Raman measurements. The investigational results showed that the variations between the spectra measured with different laser powers are almost negligible, facilitating the quantitative analysis of tissue Raman measurements in vivo.

Citation Download Citation

Shiyamala Duraipandian, Mads Bergholt, Wei Zheng, and Zhiwei Huang "Quantitative fiber-optic Raman spectroscopy for tissue Raman measurements", Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390V (4 March 2014); https://doi.org/10.1117/12.2039576

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available