Detection Of Three Rotational Correlation Times For A Rigid Asymmetric Molecule Using Frequency-Domain Fluorometry

lgnacy Gryczynski; Henryk Cherek; Joseph R Lakowicz

doi:10.1117/12.945402

24 June 1988 Detection Of Three Rotational Correlation Times For A Rigid Asymmetric Molecule Using Frequency-Domain Fluorometry

lgnacy Gryczynski, Henryk Cherek, Joseph R Lakowicz

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945402
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

We measured the frequency-response of the polarized emission of Yt-base in propylene glycol at 10°C. Data were obtained for excitation wavelengths of 290, 312 and 346 nm, for which the fundamental anisotropies are 0.05, 0.19 and 0.32, respectively. Additionally, data were obtained using CC14, to decrease the mean decay time from 9.1 to 4.2 ns. These data were analyzed globally to recover the anisotropy decay law. Three correlation times were needed to fit the data, 0.8, 3.0 and 5.6 ns, a range of only 7-fold. We believe this is the first reported detection of three correlation times for a rigid molecule.

Citation Download Citation

lgnacy Gryczynski, Henryk Cherek, and Joseph R Lakowicz "Detection Of Three Rotational Correlation Times For A Rigid Asymmetric Molecule Using Frequency-Domain Fluorometry", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945402

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