Picosecond Fluorescence Studies Of Polypeptide Dynamics

Lin X.-Q. Chen; Jacob W Petrich; Angelo Perico; Graham R Fleming

doi:10.1117/12.945391

24 June 1988 Picosecond Fluorescence Studies Of Polypeptide Dynamics

Lin X.-Q. Chen, Jacob W Petrich, Angelo Perico, Graham R Fleming

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945391
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Fluorescence anisotropies for single tryptophan-containing polypeptide hormones ACTH and glucagon, and a series of their fragments are studied. The data are discussed in the context of the theory of Perico and Guenza (J. Chem. Phys. 84, 510 (1986)) and a persistence length of about 7 to 10 residues obtained for the mobilities in the two hormones. Theory is able to account for chain length and probe location effects very well. The observed discrepencies between calculated and measured anisotropy decays very likely arise from approximations made in the calculation and from the limited time resolution of the experiments.

Citation Download Citation

Lin X.-Q. Chen, Jacob W Petrich, Angelo Perico, and Graham R Fleming "Picosecond Fluorescence Studies Of Polypeptide Dynamics", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945391

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